RT Journal Article T1 Antioxidant activity and characterization of whey protein-based beverages: Effect of shelf life and gastrointestinal transit on bioactivity A1 Arranz Gutiérrez, Elena María A1 Corrochano, Alberto A1 Shanahan, Colm A1 Villalva, Marisol A1 Jaime, Laura A1 Santoyo, Susana A1 Callanan, Michael A1 Murphy, Eoin A1 Giblin, Linda AB Whey proteins can exhibit antioxidant activity. The objectives of this study were to formulate model whey based beverages with well-established antioxidants (plant polyphenols, vitamins and astaxanthin) to investigate (1) the antioxidant shelf life over a 24-week period and (2) the antioxidant activity after upper gastrointestinal transit. Pilot scale processing (pasteurization, ultra-high temperature or spray drying) was used to prepare beverages which were representative of current product formats. In vitro gastrointestinal digestion of test samples was performed using the standardised INFOGEST method and antioxidant activity of samples was determined using ABTS, FRAP and ORAC. Results from the antioxidant shelf life study provided evidence that powder products functionality was preserved. Whey beverages (pasteurised or spray dried) increased or maintained antioxidant activity during gastrointestinal transit. Combination of whey with additional antioxidant ingredients increased the bioactivity of formulated products; however, this greater bioactivity was altered after gastrointestinal transit, depending on processing type and antioxidant methodology. PB Elsevier SN 1466-8564 YR 2019 FD 2019 LK https://hdl.handle.net/20.500.14352/93909 UL https://hdl.handle.net/20.500.14352/93909 LA eng NO Arranz, E., Corrochano, A. R., Shanahan, C., Villalva, M., Jaime, L., Santoyo, S., ... & Giblin, L. (2019). Antioxidant activity and characterization of whey protein-based beverages: Effect of shelf life and gastrointestinal transit on bioactivity. Innovative Food Science & Emerging Technologies, 57, 102209. NO Irish Department of Agriculture, Food and the Marine NO Enterprise Ireland NO European Commission DS Docta Complutense RD 24 abr 2025