RT Journal Article
T1 Authentication of commercial candy ingredients using DNA PCR-cloning methodology
A1 Muñoz Colmenero, Ana Marta
A1 Martínez, Jose Luis
A1 Roca, Agustín
A1 García Vázquez, Eva
AB BACKGROUNDCommercial candies are consumed by all population age sectors worldwide. Methods for quality control and composition authentication are therefore needed for best compliance with consumers' preferences. In this study applications of DNA-based methodology for candy quality control have been tested. Eighteen samples of commercial candies (marshmallows, gumdrops, jelly, sherbet, gelatin-based desserts) produced by five countries were analyzed to identify the component species by polymerase chain reaction, cloning and sequencing of 16S rRNA and ribulose −1,5-diphosphate carboxylase oxygenase genes, and the species determined from BLAST comparison with universal databases and phylogenetic analysis.RESULTSPositive DNA extraction and amplification of the target genes were obtained for 94% of candies assayed, even those containing as little as <0.0005 ng µL−1 DNA concentration. The results demonstrated that the species detected from DNA were compatible with the information provided on candy labels only in a few products. DNA traces of undeclared species, including fish, were found in most samples, and two products were labeled as vegetarian but contained porcine DNA.CONCLUSIONBased on the inaccuracy found on the labels of sweets we recommend the use of DNA tests for quality control of these popular sweets. DNA tests have been useful in this field but next-generation sequencing methods could be more effective. © 2015 Society of Chemical Industry
PB Wiley
SN 0022-5142
YR 2016
FD 2016-02
LK https://hdl.handle.net/20.500.14352/109577
UL https://hdl.handle.net/20.500.14352/109577
LA eng
NO Muñoz‐Colmenero M, Martínez JL, Roca A, García‐Vázquez E. Authentication of commercial candy ingredients using DNA PCR ‐cloning methodology. J Sci Food Agric 2016;96:859–67. https://doi.org/10.1002/jsfa.7158.
NO MM-C holds a Spanish National Grant (reference AP-2010-5211)
NO Principado de Asturias
NO Universidad de Oviedo
NO Fundación Mapfre
DS Docta Complutense
RD 7 abr 2025