RT Journal Article
T1 Evidence of the Beneficial Impact of Three Probiotic-Based Food Supplements on the Composition and Metabolic Activity of the Intestinal Microbiota in Healthy Individuals: An Ex Vivo Study
A1 Sánchez Beltrán, María Del Carmen
A1 Herráiz, Ana
A1 Tigre, Sindy
A1 Llama Palacios, María Arantxazu
A1 Hernández, Marta
A1 Ciudad Cabañas, María José
A1 Collado Yurrita, Luis Rodolfo
AB Scientific evidence has increasingly supported the beneficial effects of probiotic-based food supplements on human intestinal health. This ex vivo study investigated the effects on the composition and metabolic activity of the intestinal microbiota of three probiotic-based food supplements, containing, respectively, (1) Bifidobacterium longum ES1, (2) Lactobacillus acidophilus NCFM®, and (3) a combination of L. acidophilus NCFM®, Lactobacillus paracasei Lpc-37™, Bifidobacterium lactis Bi-07™, and Bifidobacterium lactis Bl-04™. This study employed fecal samples from six healthy donors, inoculated in a Colon-on-a-plate® system. After 48 h of exposure or non-exposure to the food supplements, the effects were measured on the overall microbial fermentation (pH), changes in microbial metabolic activity through the production of short-chain and branched-chain fatty acids (SCFAs and BCFAs), ammonium, lactate, and microbial composition. The strongest effect on the fermentation process was observed for the combined formulation probiotics, characterized by the significant stimulation of butyrate production, a significant reduction in BCFAs and ammonium in all donors, and a significant stimulatory effect on bifidobacteria and lactobacilli growth. Our findings suggest that the combined formulation probiotics significantly impact the intestinal microbiome of the healthy individuals, showing changes in metabolic activity and microbial abundance as the health benefit endpoint.
PB MDPI
SN 2072-6643
YR 2023
FD 2023-12-12
LK https://hdl.handle.net/20.500.14352/103910
UL https://hdl.handle.net/20.500.14352/103910
LA eng
NO Sánchez, M.C.; Herráiz, A.; Tigre, S.; Llama-Palacios, A.; Hernández, M.; Ciudad, M.J.; Collado, L. Evidence of the Beneficial Impact of Three Probiotic-Based Food Supplements on the Composition and Metabolic Activity of the Intestinal Microbiota in Healthy Individuals: An Ex Vivo Study. Nutrients 2023, 15, 5077. https:// doi.org/10.3390/nu15245077
NO Author Contributions: Protocol design, M.C.S., M.J.C. and M.H.; methodology, M.C.S., A.H. and A.L.-P.; formal analysis and investigation, M.C.S., A.H. and S.T.; data curation, M.C.S., A.H. and S.T.; writing—original draft preparation, M.C.S., A.H. and S.T.; writing—review and editing, M.J.C., L.C. and M.H. All authors have read and agreed to the published version of the manuscript.
DS Docta Complutense
RD 9 abr 2025