RT Journal Article
T1 Nanocrystallinity effects on osteoblast and osteoclast response to silicon substituted hydroxyapatite
A1 Casarrubios Molina, Laura
A1 Matesanz Sancho, María Concepción
A1 Sánchez Salcedo, Sandra
A1 Arcos Navarrete, Daniel
A1 Vallet Regí, María Dulce Nombre
A1 Portolés Pérez, María Teresa
AB Hypothesis: Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200ºC) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700ºC) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two  main cell types involved in bone remodelling) to silicon substituted hydroxyapatite.Experiments: Saos-2 osteoblasts and osteoclast-like cells (differentiated from RAW-264.7 macrophages)have been cultured on the surface of nano-SiHA and SiHA disks and different cell parameters have been evaluated: cell adhesion, proliferation, viability, intracellular content of reactive oxygen species, cell cycle phases, apoptosis, cell morphology, osteoclast-like cell differentiation and resorptive activity. Findings: This comparative in vitro study evidences that nanocrystallinity of SiHA affects the cell/biomaterial interface inducing bone cell apoptosis by loss of cell anchorage (anoikis), delaying osteoclast-like cell differentiation and decreasing the resorptive activity of this cell type. These results suggest the potential use of nano-SiHA biomaterial for preventing bone resorption in treatment of osteoporotic bone.
PB Elsevier
SN 0021-9797
YR 2016
FD 2016-11-15
LK https://hdl.handle.net/20.500.14352/17594
UL https://hdl.handle.net/20.500.14352/17594
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID N-4501-2014  (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
NO Ministerio de Ciencia e Innovacion (MICINN)
NO Ministerio de Economia y Competitividad (MINECO)
NO Agening Network of Excellence
DS Docta Complutense
RD 6 abr 2025