RT Journal Article
T1 Active surveillance of antimicrobial resistance in companion animals: A pilot study in a Spanish Veterinary Teaching Hospital
A1 Scarpellini, Raffaele
A1 Leal Vélez de Mendizábal, Laura
A1 Quevedo Caraballo, Sergio
A1 Blanco Cancelo, José Luis
A1 García Sánchez, Marta Eulalia
A1 Pérez Sancho, Marta
A1 Portero Fuentes, Miriam
A1 Penelo Hidalgo, Silvia
A1 Esposito, Erika
A1 Mondo, Elisabetta
A1 Piva, Silvia
AB The role of small animal veterinary hospitals in the onset and dissemination of antimicrobial-resistant organisms (AMROs) is still not clear, and the implementation of an internal surveillance systems is a cost-effective tool to better understand their impact. The aim of this study was to describe a pilot program of active surveillance in a Spanish Veterinary Teaching Hospital, developed to estimate the detection frequency of AMROs in the commensal flora of patients and in the environment. Surveillance was focused on Methicillin-resistant Staphylococci (MRS), third generation cephalosporins resistant gram-negative bacteria (3GCR-GNB), and carbapenems-resistant gram-negative bacteria (CR-GNB). Oral and perirectal swabs were collected in the same dogs and cats hospitalized > 48 h, at their admission and before their discharge. Out of 50 patients sampled, 24% (12/50) were carriers at admission of at least one of the three investigated AMROs. Twenty-eight percent of patients (14/50) acquired at least one AMRO during the hospital stay. MRS detection frequency at admission was 12% (6/50), while acquisition was 6% (3/50). 3GCR-GNB detection frequency was 14% at admission (7/50) and acquisition 22% (11/50), while CR-GNB detection frequency was 2% at admission (1/50) and acquisition 2% (1/50). Environmental surveillance (98 samples) showed a total detection frequency of 22.4% for MRS (22/98), 2% for 3GCR-GNB and CR-GNB (2/98). Clinical staff’ shoe soles showed high detection frequency for MRS (50%). 3GCR Escherichia coli was the most isolated species in patients (n = 17). The results show how active surveillance can be used as a tool to assess the impact of AMROs in veterinary hospitals to subsequently build up tailored control plans based on specific issues.
PB Elsevier
SN 0147-9571
YR 2024
FD 2024-04-02
LK https://hdl.handle.net/20.500.14352/103273
UL https://hdl.handle.net/20.500.14352/103273
LA eng
NO Abbreviations: AMR, antimicrobial resistance; AMRO, antimicrobial-resistant organisms; AST, antimicrobial susceptibility testing; CDT, combination disc test; CRGNB, carbapenem-resistant gram-negative bacteria; ESBL-E, extended spectrum beta-lactamases producing Enterobacterales; ESCMID, European Society of Clinical Microbiology and Infectious Diseases; EUCAST, European Committee of Antimicrobial Suceptibility Testing; HCAI, healthcare-associated infections; MALDI-TOF, matrix-assisted laser desorption/ionization time of flight; MRS, methicillin-resistant Staphylococci; MRSA, methicillin-resistant Staphylococcus aureus; MRSP, methicillin-resistant Staphylococcus pseudintermedius; 3GCR-GNB, 3rd generation cephalosporins-resistant gram-negative bacteria; VTH, veterinary teaching hospital. Authorship contribution statement: Marta Perez Sancho: Writing – review & editing, Visualization, Validation, Project administration, Methodology, Formal analysis, Data curation. Miriam Portero Fuentes: Visualization, Resources, investigation. Jose Luis Blanco: Writing – review & editing, Visualization, Validation, Supervision, Resources, Project administration, Formal analysis, Conceptualization. Marta Eulalia Garcia: Writing – review & editing, Supervision, Resources, Project administration, Conceptualization. Laura Leal Velez De Mendizabal: ´ Writing – review & editing, Methodology, Investigation. Sergio Quevedo Caraballo: Investigation. Silvia Piva: Writing – review & editing, Validation, Supervision, Resources, Funding acquisition. Raffaele Scarpellini: Writing – review & editing, Writing – original draft, Project administration, Methodology, Investigation, Data curation, Conceptualization. Erika Esposito: Datacuration, Conceptualization. Elisabetta Mondo: Visualization. Silvia Penelo: Resources, Investigation.
DS Docta Complutense
RD 10 sept 2024