RT Journal Article
T1 Toxicogenomics in a soil sentinel exposure to Zn nanoparticles and ions reveals the comparative role of toxicokinetic and toxicodynamic mechanisms
A1 Novo Rodríguez, Marta
A1 Lahive, Elma
A1 Díez Ortiz, María
A1 Spurgeon, David J.
A1 Kille, Peter
AB A critical question for read across of the hazards of nanomaterials is the degree to which their mechanisms of action differ from those of their bulk chemical constituents. It has been established that metal and metal oxide nanoparticles (NPs) can be accumulated by invertebrate species. Moreover, it has been hypothesised that the observed toxicity resulting on exposure to these nanomaterials is most likely to be associated with the releases of ions from external or internal dissolution leading ultimately to toxicity. However, mechanistic confirmation of the similar modes of action for metal oxide nanomaterials and metal ions in studies invertebrates are largely lacking. Therefore, here we present a toxicogenomic study using exposed individuals of the earthworm Eisenia fetida from a single genetic lineage. We compared gene expression and pathway responses through RNA-seq analysis at equitoxic concentrations (EC50 for reproduction) of ZnO NPs and ionic Zn. We found similar transcriptomic effects for both Zn forms with genetic signatures of tight control of cytosolic Zn concentrations through expression changes of genes encoding several Zn transporters. Activation and regulation of JUN, MAP and JNK kinases indicated a cellular response to the increased Zn concentrations of both forms with compound binding terms also enriched. Our results illustrate the need to consider both toxicokinetic and toxicodynamic mechanisms in the development of adverse outcome pathways for nanomaterials.
PB Royal Society of Chemistry
SN 2051-8153
YR 2020
FD 2020-03-20
LK https://hdl.handle.net/20.500.14352/115926
UL https://hdl.handle.net/20.500.14352/115926
LA eng
NO Novo M, Lahive E, Díez Ortiz M, Spurgeon DJ, Kille P. Toxicogenomics in a soil sentinel exposure to Zn nanoparticles and ions reveals the comparative role of toxicokinetic and toxicodynamic mechanisms. Environmental Science: Nano 7: 1464 (2020). https://doi.org/10.1039/C9EN01124B
NO MN and MD were supported by Marie Curie Fellowships, FP7-IEF-GA-2012-329690, FP7-IEF-GA-2010-273207. MN was also supported by a Postdoctoral Fellowship from Complutense University. Remaining authors were supported by NanoFATE, Project-CP-FP-247739 under FP7-NMP-ENV-2009, Theme-4; www. nanofate.eu. DJS and EL were supported by the UKRI IMP scheme project NEC06896.
NO European Commission
NO Universidad Complutense de Madrid
NO UK Research and Innovation
DS Docta Complutense
RD 9 abr 2025