%0 Journal Article %A Khodarahmi, Reza %A Torrente Rodríguez, Rebeca Magnolia %A Ruiz-Valdepeñas Montiel, Víctor %A Campuzano Ruiz, Susana %A Pedrero Muñoz, María %A Farchado, Meryem %A Vargas, Eva %A Manuel De Villena Rueda, Francisco Javier %A Garranzo Asensio, María %A Barderas Manchado, Rodrigo %A Pingarrón Carrazón, José Manuel %T Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates %D 2017 %@ 1932-6203 %U https://hdl.handle.net/20.500.14352/18048 %X The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates. %~