RT Journal Article
T1 Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates
A1 Khodarahmi, Reza
A1 Torrente Rodríguez, Rebeca M.
A1 Ruiz-Valdepeñas Montiel, Víctor
A1 Campuzano, Susana
A1 Pedrero Muñoz, María
A1 Farchado, Meryem
A1 Vargas, Eva
A1 Manuel de Villena, F. Javier
A1 Garranzo Asensio, María
A1 Barderas, Rodrigo
A1 Pingarrón Carrazón, José Manuel
AB The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.
PB San Francisco, CA : Public Library of Science
SN 1932-6203
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18048
UL https://hdl.handle.net/20.500.14352/18048
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 3 may 2024