%0 Journal Article %A Prem, Deepak %A Bárány, Ivett %A Rodríguez-Sanz, Héctor %A Risueño, Mª del Carmen %A Testillano, Pilar %A Solís González, María Teresa %T A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus %D 2012 %@ 1471-2229 %U https://hdl.handle.net/20.500.14352/95748 %X Microspore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolated microspores at 32°C is considered to be a pre-requisite for embryogenesis induction. %~