RT Journal Article T1 Red pitaya (Hylocereus costaricensis) peel as a source of valuable molecules: Extraction optimization to recover natural colouring agents. A1 Roriz, Custodio Lobo A1 Heleno, Sandrina A. A1 Oliveira, Beatriz P.P. A1 Pinela, José A1 Dias, Maria Inês A1 Calhelha, Ricardo R. A1 Morales Gómez, Patricia A1 Ferreira, Isabel C.F.R. A1 Barros, Lillian AB Hylocereus costaricensis peel contains large amounts of betacyanins and can be exploited as a source of natural colorants. This work aimed the chemical characterization and evaluation of bioactive properties of this by-product and the optimization of the ultrasound-assisted extraction (UAE) of betacyanins using the response surface methodology (RSM). Oxalic and malic acids and traces of fumaric acid were detected, as well as the four tocopherol isoforms, predominantly γ-tocopherol. Four betacyanins were identified and used as response criteria for UAE optimization, namely phyllocactin, isobetanin, isophyllocactin, and betanin. Sample processing at 487 W for 38 min result in the maximum betacyanin content (36 ± 1 mg/g dw). The peel extract inhibit the oxidative haemolysis, with IC50 values of 255 and 381 μg/mL for Δt of 60 and 120 min, respectively, and the growth of pathogenic bacteria, with minimum inhibitory concentrations ranging from 5 to 20 mg/mL. Furthermore, no toxicity was observed for normal cells. PB Elsevier SN 0308-8146 YR 2022 FD 2022 LK https://hdl.handle.net/20.500.14352/93481 UL https://hdl.handle.net/20.500.14352/93481 LA eng NO Roriz CL, Heleno SA, Alves MJ, Oliveira MBPP, Pinela J, Dias MI, et al. Red pitaya (Hylocereus costaricensis) peel as a source of valuable molecules: Extraction optimization to recover natural colouring agents. Food Chemistry 2022;372:131344. https://doi.org/10.1016/j.foodchem.2021.131344. NO Foundation for Science and Technology ( Portugal) NO European Commission NO Universidad Complutense de Madrid DS Docta Complutense RD 12 abr 2025