RT Journal Article
T1 Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications
A1 Amorim, Adriany das Graças Nascimento
A1 Sánchez-Paniagua López, Marta
A1 Oliveira, Taiane Maria de
A1 Mafud, Ana Carolina
A1 Alves da Silva, Durcilene
A1 Souza de Almeida Leite, José Roberto de
A1 López Ruiz, María Beatriz
AB This research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between –NH and –OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 53 nm and a polydispersity index of 0.309 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 mA mM1 cm2 ), wide linear range of 2.5   107 –2.0   104 M, and low detection limit (50 nM).
SN 2050-750X
YR 2021
FD 2021-08-02
LK https://hdl.handle.net/20.500.14352/99976
UL https://hdl.handle.net/20.500.14352/99976
LA eng
NO Das Graças Nascimento Amorim A, Sánchez-Paniagua M, De Oliveira TM, Mafud AC, Da Silva DA, De Souza De Almeida Leite JR, et al. Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications. J Mater Chem B 2021;9:6825–35. https://doi.org/10.1039/D1TB01164B.
NO Universidad Complutense de Madrid
NO Federal University of Piaui (Brasil)
DS Docta Complutense
RD 8 abr 2025