RT Journal Article
T1 Alteration of medial-edge epithelium cell adhesion in two Tgf-beta3 null mouse strains
A1 Martínez Sanz, Elena
A1 Río Sevilla, Aurora Del
A1 Barrio Asensio, María Del Carmen
A1 Maldonado Bautista, Estela
A1 Murillo González, Jorge Alfonso
A1 Garcillán, B
A1 Amorós, M
A1 Fuerte, T
A1 Fernández, A
A1 Trinidad, E
A1 Rabadán, MA
A1 López, Y
A1 Martínez Salmeán, María Luisa
A1 Martínez Álvarez, María Concepción
AB Although palatal shelf adhesion is a crucial event during palate development, little work has been carried out to determine which molecules are responsible for this process. Furthermore, whether altered palatal shelf adhesion causes the cleft palate presented by Tgf-b3 null mutant mice has not yet been clarified. Here, we study the presence/distribution of some extracellular matrix and cell adhesion molecules at the time of the contact of palatal shelves in both wild-type and Tgf-b3 null mutant palates of two strains of mice (C57/BL/6J(C57), and MF1) that develop cleft palates of different severity. We have performed immunohistochemistry with antibodies against collagens IV and IX, laminin, fibronectin, the a5- and b1-integrins, and ICAM-1; in situ hybridization with a Nectin-1 riboprobe; and palatal shelf cultures treated or untreated with TGF-b3 or neutralizing antibodies against fibronectin or the a5-integrin. Our results show the location of these molecules in the wild-type mouse medial edge epithelium (MEE) of both strains at the time of the contact of palatal shelves; the heavier (C57) and milder (MF1) alteration of their presence in the Tgf-b3 null mutants; the importance of TGF-b3 to restore their normal pattern of expression; and the crucial role of fibronectin and the a5-integrin in palatal shelf adhesion. We thus provide insight into the molecular bases of this important process and the cleft palate presented by Tgf-b3 null mutant mice.
PB Elsevier
SN 0301-4681
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/101454
UL https://hdl.handle.net/20.500.14352/101454
LA eng
NO Martínez-Sanz, E., Del Río, A., Barrio, C., Murillo, J., Maldonado, E., Garcillán, B., ... & Martínez-Álvarez, C. (2008). Alteration of medial-edge epithelium cell adhesion in two Tgf-β3 null mouse strains. Differentiation, 76(4), 417-430.
NO Fondo de Investigaciones Sanitarias
NO Comunidad Autónoma de Madrid
NO Universidad Complutense
DS Docta Complutense
RD 8 abr 2025