%0 Journal Article
%A Álvarez Gómez De Segura, Ignacio
%A Calvo, Luis
%A Óvilo, Cristina
%A González-Bulnes, Antonio
%A Olivares Moreno, Álvaro
%A Cambero Rodríguez, María Isabel
%A López Bote, Clemente José
%T Alternative method for intramuscular fat analysis using common laboratory equipment
%D 2015
%@ 0309-1740
%U https://hdl.handle.net/20.500.14352/94018
%X A procedure to quantify intramuscular fat was developed using common inexpensive laboratory equipment. Three homogenization methods of lyophilized muscle samples (Ball-mill, Grinder and Mortar) and two extraction methods (Ball-mill or Vortex) were used in turkey meat and pork. Two-hundred mg of lyophilized and homogenized samples were accurately weighed and mixed with 1.5 mL of dichloromethane-methanol (8:2) and shaken either in a Mixer Mill (MM400, Retsch Technology) or in a Vortex. The final mixture was separated by centrifugation. Solvent was evaporated under a nitrogen stream and lipid content was gravimetrically determined. Besides, it was checked that the fatty acid profile was not altered by the protocol used. Moreover, the analysis of 4 replicas from the same sample showed different variation coefficients (16-29%) for the new procedures proposed over a wide range of IMF content. The combination of Grinder and Vortex methodologies can be proposed as a simple and inexpensive alternative to previous ones.
%~