RT Journal Article
T1 11PS04 is a new chemical entity identified by microRNA-based biosensing with promising therapeutic potential against cancer stem cells
A1 Aguado Sánchez, Tania
A1 Romero-Revilla, José
A1 Granados, Rosario
A1 Campuzano Ruiz, Susana
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Cuesta Martínez, Ángel
A1 Albiñana, Virginia
A1 Botella, Luisa María
A1 Santamaría, Silvia
A1 Garcia-Sanz, Jose
A1 Pingarrón Carrazón, José Manuel
A1 Sánchez-Sancho, Francisco
A1 Sánchez-Puelles, José-María
AB Phenotypic drug discovery must take advantage of the large amount of clinical data currently available. In this sense, the impact of microRNAs (miRs) on human disease and clinical therapeutic responses is becoming increasingly well documented. Accordingly, it might be possible to use miR-based signatures as phenotypic read-outs of pathological status, for example in cancer. Here, we propose to use the information accumulating regarding the biology of miRs from clinical research in the preclinical arena, adapting it to the use of miR biosensors in the earliest steps of drug screening. Thus, we have used an amperometric dual magnetosensor capable of monitoring a miR-21/miR-205 signature to screen for new drugs that restore these miRs to non-tumorigenic levels in cell models of breast cancer and glioblastoma. In this way we have been able to identify a new chemical entity, 11PS04 ((3aR,7aS)-2-(3-propoxyphenyl)-7,7a-dihydro-3aH-pyrano[3,4-d]oxazol-6(4H)-one), the therapeutic potential of which was suggested in mechanistic assays of disease models, including 3D cell culture (oncospheres) and xenografts. These assays highlighted the potential of this compound to attack cancer stem cells, reducing the growth of breast and glioblastoma tumors in vivo. These data demonstrate the enhanced chain of translatability of this strategy, opening up new perspectives for drug-discovery pipelines and highlighting the potential of miR-based electro-analytical sensors as efficient tools in modern drug discovery.
PB Nature Research
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/91612
UL https://hdl.handle.net/20.500.14352/91612
LA eng
NO Aguado, T., Romero-Revilla, J.A., Granados, R. et al. 11PS04 is a new chemical entity identified by microRNA-based biosensing with promising therapeutic potential against cancer stem cells. Sci Rep 9, 11916 (2019). https://doi.org/10.1038/s41598-019-48359-y
NO Ministerio de Economía, Industria y Competitividad (España)
NO Comunidad de Madrid 
NO Instituto de Salud Carlos III 
NO Centro de Investigación Biomédica en Red de Enfermedades Respiratorias 
NO European Commission
DS Docta Complutense
RD 13 abr 2025