RT Journal Article
T1 Dual Internal Standards with Metals and Molecules for MALDI Imaging of Kidney Lipids
A1 Aboulmagd, Sarah
A1 Esteban Fernández, Diego
A1 Moreno Gordaliza, María Estefanía
A1 Neumann, Boris
A1 El-Khatib, A.H.
A1 Lázaro Fernández, Alberto
A1 Tejedor Jorge, Alberto
A1 Gómez Gómez, María Milagros
A1 Linscheid, Michael W.
AB The quest for internal standards useful in MALDI imaging studies goes on to get not only lateral distribution but also reliable relative quantitative information. We developed a method based on application of matrix and dual internal standards to allow intra- and intersample normalization of lipids intensities in kidney sections of control and cisplatin-treated Wistar rats. An inkjet printer was used to deposit a custom-prepared ink with DHB as MALDI matrix, a primary lipids-based internal standard, and a spiked lanthanide as a secondary internal standard. We applied different laser energy and varied the amounts of matrix-internal standards mixture to evaluate the normalization potential of the internal standards. Successful correction of intensity artifacts caused by instrumental drifts was possible, but not those resulting from uneven matrix application. ICP-MS absolute quantification of the lanthanide in the printed layer ensured the reproducibility of the matrix and internal standards application with RSD of 10−15%. Internal standard-normalized data allowed intrasample modification of the MALDI image to make it compatible with the optical image. Normalization to internal standards corrected a 2-fold difference in lipids intensity, which allowed a meaningful comparison of tissue lipids in control and cisplatin-treated kidneys. More importantly, normalization of lipid relative abundances based on the same adduct type (H+ , Na+ , and K+ ) for analyte and internal standard corrected for different ionization efficiencies showing a realistic signal level and enabling reliable comparison of different samples on relative quantitative basis.
PB ACS
SN 0003-2700
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18777
UL https://hdl.handle.net/20.500.14352/18777
LA eng
NO Aboulmagd, S., Esteban Fernández, D., Moreno Gordaliza, E. et al. «Dual Internal Standards with Metals and Molecules for MALDI Imaging of Kidney Lipids». Analytical Chemistry, vol. 89, n.o 23, diciembre de 2017, pp. 12727-34. DOI.org (Crossref), https://doi.org/10.1021/acs.analchem.7b02819.
DS Docta Complutense
RD 9 abr 2025