RT Journal Article
T1 Protective effects of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) against oxidant-induced stress in human colon carcinoma Caco-2 cells
A1 Martínez Caballero, María Aranzazu
A1 Ares Lombán, Irma
A1 Martínez Caballero, Marta
A1 López Torres, Bernardo
A1 Rodríguez, José Luis
A1 Maximiliano Guerra, Jorge Enrique
A1 Martínez Larrañaga, María Rosa
A1 Anadón Navarro, Arturo Ramón
A1 Rosa, José Manuel de la
A1 Cueto, Mercedes
AB The present study investigated the effect of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) on cell viability and the potential protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butyl hydroperoxide (t-BOOH). Both extracts prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with t-BOOH. Increased ROS and caspase 3/7 activity induced by t-BOOH were dose-dependently prevented when cells were treated with the extracts. CB08035-SCA caused up-regulation of Nrf2, AKT1 and Bcl-2 gene expressions. Moreover, CB08035-SCA and CB08035-SYP treatments reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions of apoptosis, proinflammation and oxidative stress induced by t-BOOH. CB08035-SCA and CB08035-SYP CPE extracts confer a significant protection against oxidative insults to cells. Our results show that culture extracts CB08035-SCA and CB08035-SYP from M. hydrocarbonoclasticus (strain CB08035) appeared to have antioxidant potential, based on their ability to protect antioxidant enzymes and mRNA gene expressions linked to apoptosis/oxidative pathways. These results suggest that culture extracts CB08035-SCA and CB08035-SYP can be a potential ingredient in the pharmaceutical and cosmeceutical industries.
PB Elsevier
SN 0278-6915
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/97616
UL https://hdl.handle.net/20.500.14352/97616
LA eng
NO Martínez MA, Ares I, Martínez M, Lopez-Torres B, Rodríguez JL, Maximiliano JE, Martínez-Larrañaga MR, Anadón A, Rosa JM, Cueto M. Protective effects of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) against oxidant-induced stress in human colon carcinoma Caco-2 cells. Food Chem Toxicol. 2020 Nov;145:111671. doi: 10.1016/j.fct.2020.111671. Epub 2020 Aug 9. PMID: 32784000.
NO Credit authorship contribution statementMaría-Aránzazu Martínez: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation, Writing - review & editing. Irma Ares: Investigation, Formal analysis, Writing - original draft, Writing - review & editing. Marta Martínez: Investigation, Formal analysis, Writing - original draft, Writing - review & editing. Bernardo Lopez-Torres: Methodology, Investigation. Jose-Luis Rodríguez: Methodology, Investigation, Formal analysis. Jorge-Enrique Maximiliano: Methodology, Investigation. María-Rosa Martínez-Larrañaga: Conceptualization, Methodology, Investigation, Formal analysis. Arturo Anadón: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Jose-Manuel de la Rosa: Methodology, Investigation. Mercedes Cueto: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation, Writing - review & editing.
NO Ministerio de Economía, Industria y Competitividad (España)
DS Docta Complutense
RD 7 abr 2025