RT Journal Article
T1 Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur
A1 Rebollada Merino, Agustín Miguel
A1 Vela Alonso, Ana Isabel
A1 Canales, Rocío
A1 Romani Cremaschi, Umberto
A1 Ugarte Ruiz, María
A1 Buendía, Arantxa
A1 Pérez Sancho, Marta
A1 Domínguez Rodríguez, Lucas José
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Rodríguez Bertos, Antonio Manuel
AB Lactococci have been associated with fetal and neonatal infections in humans and cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri in a lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead in the indoor area of a zoologic exhibit. Classification as a late-term abortion or stillbirth was unclear as the precise gestational time was unknown. A medical checkup of the dam revealed fever and neutrophilic leukocytosis; recovery followed treatment with enrofloxacin. The main histologic findings were placental edema and hemorrhage, hepatic necrosis, desquamated amniotic epithelial cells in alveoli, and subendocardial and myocardial hemorrhages. Tissue Gram stain revealed abundant gram-positive cocci arranged in short chains in the placenta and liver. Toxoplasma gondii was not detected by immunohistochemistry. Bacterial isolates from the placenta and fetal liver were identified as Lactococcus garvieae by MALDI-TOF MS. However, the isolates were found to be L. petauri by determining their in-silico DNA–DNA hybridization and average nucleotide identity values using pairwise comparisons of their whole-genome sequences and the genomes of the type strains. The antimicrobial susceptibility of isolates by the disk diffusion method revealed resistance to tylosin, gentamicin, apramycin, neomycin, amikacin, ampicillin, and florfenicol. We attributed the reproductive loss in this lemur to placental and fetal infection by L. petauri.
PB Sage Journals
SN 1040-6387
YR 2025
FD 2025-02-28
LK https://hdl.handle.net/20.500.14352/118665
UL https://hdl.handle.net/20.500.14352/118665
LA eng
NO Rebollada-Merino, A., Vela, A. I., Canales, R., Romani-Cremaschi, U., Ugarte-Ruiz, M., Buendía, A., Pérez-Sancho, M., Domínguez, L., Fernández-Garayzabal, J. F., & Rodríguez-Bertos, A. (2025). Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 10406387251323565. Advance online publication. https://doi.org/10.1177/10406387251323565
DS Docta Complutense
RD 4 abr 2025