RT Journal Article
T1 Influence of post-mortem sperm recovery method and extender on unstored and refrigerated rooster sperm variables
A1 Villaverde Morcillo, Silvia
A1 Esteso Díez, Milagros Cristina
A1 Castaño García, Cristina
A1 Santiago Moreno, Julián
AB Many post-mortem sperm collection techniques have been described for mammalian species, but their use in birds is scarce. This paper compares the efficacy of two post-mortem sperm retrieval techniques - the flushing and float-out methods - in the collection of rooster sperm, in conjunction with the use of two extenders, i.e., L&R-84 medium and Lake 7.1 medium. To determine whether the protective effects of these extenders against refrigeration are different for post-mortem and ejaculated sperm, pooled ejaculated samples (procured via the massage technique) were also diluted in the above extenders. Post-mortem and ejaculated sperm variables were assessed immediately at room temperature (0 h), and after refrigeration at 5°C for 24 and 48 h. The flushing method retrieved more sperm than the float-out method (596.5 ± 75.4 million sperm vs 341.0 ± 87.6 million sperm; p < 0.05); indeed, the number retrieved by the former method was similar to that obtained by massage-induced ejaculation (630.3 ± 78.2 million sperm). For sperm collected by all methods, the L&R-84 medium provided an advantage in terms of sperm motility variables at 0 h. In the refrigerated sperm samples, however, the Lake 7.1 medium was associated with higher percentages of viable sperm, and had a greater protective effect (p < 0.05) with respect to most motility variables. In conclusion, the flushing method is recommended for collecting sperm from dead birds. If this sperm needs to be refrigerated at 5°C until analysis, Lake 7.1 medium is recommended as an extender.
PB Wiley
SN 0936–6768
YR 2015
FD 2015-11-24
LK https://hdl.handle.net/20.500.14352/116320
UL https://hdl.handle.net/20.500.14352/116320
LA eng
NO Villaverde-Morcillo, S., Esteso, M., Castaño, C. and Santiago-Moreno, J. (2016), Influence of Post-Mortem Sperm Recovery Method and Extender on Unstored and Refrigerated Rooster Sperm Variables. Reprod Dom Anim, 51: 40-46. https://doi.org/10.1111/rda.12643
NO Author contributions:S. Villaverde-Morcillo: performed the semen recovery and analysis; analysed the data; writing the basis of the article. M.C. Esteso: conception and design of the study; discussed the data, and approved the final draft. C. Castaño: worked on animal management, semen collection and analysis; approved the final draft. J. Santiago-Moreno: conception and design of the study, participation and general supervision of the collection of data, writing the basis of the article, and approved.
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
DS Docta Complutense
RD 10 abr 2025