RT Journal Article
T1 The supplementations with 2-hydroxyoleic acid and n-3 polyunsaturated fatty acids revert oxidative stress in various organs of diet-induced obese mice
A1 Hunsche, Caroline
A1 Martínez de Toda Cabeza, Irene
A1 Hernández, Oskarina
A1 Jiménez, Beatriz
A1 Díaz, Ligia Esperanza
A1 Marcos, Ascensión
A1 Fuente del Rey, Mónica de la
AB Obesity and its related diseases have been associated with oxidative stress. Thus, the search for nutritional strategies to ameliorate oxidative stress in obese individuals seems important. We hypothesized that the supplementation with monounsaturated (2-hydroxyoleic acid (2-OHOA)) and with combined n-3 polyunsaturated (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) fatty acids would ameliorate oxidative stress in different organs, including brain, liver, lungs, and kidneys of adult diet-induced obese (DIO) mice. Adult female ICR-CD1 mice were fed a high-fat diet (HFD) for 14 weeks. During the last 6 weeks of HFD feeding, one group of DIO mice received the same HFD, supplemented with 1500 mg of 2-OHOA per kg of HFD and another group with 1500 mg of EPA and 1500 mg of DHA per kg of HFD. At the end of the experiment, several parameters of oxidative stress were assessed. The supplementation with 2- OHOA or with EPA and DHA in DIO mice was able to revert oxidative stress, enhancing the activities of catalase and glutathione reductase, as well as diminishing the activity of xanthine oxidase, the concentration of thiobarbituric acid reactive substances (TBARS) and the ratio between oxidized glutathione and reduced glutathione in several organs. These reached similar values to those of control mice, which were fed a standard diet. These data suggest that supplementation with 2-OHOA and with EPA and DHA could be an effective nutritional intervention to restore an appropriate redox state in DIO mice.
PB Taylor & Francis
SN 1071-5762; Electronic: 1029-2470
YR 2020
FD 2020-08-04
LK https://hdl.handle.net/20.500.14352/7877
UL https://hdl.handle.net/20.500.14352/7877
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III/FEDER
NO Universidad Complutense de Madrid
NO PRONAOS Study
NO BTSA-Applied Biotechnologies S.L
DS Docta Complutense
RD 2 may 2024