RT Journal Article
T1 Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
A1 Ruiz Yance, Iris
A1 Siguas, Junior
A1 Bardales, Brandy
A1 Robles Castañeda, Ingrid
A1 Cordova, Karen
A1 Ypushima, Alina
A1 Estela Villar, Esteban
A1 Quintana Criollo, Carlos
A1 Estacio, Darwin
A1 Rodríguez, José Luis
AB Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1β, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis.
PB MDPI
YR 2023
FD 2023-10-05
LK https://hdl.handle.net/20.500.14352/104025
UL https://hdl.handle.net/20.500.14352/104025
LA eng
NO Ruiz-Yance, I., Siguas, J., Bardales, B., Robles-Castañeda, I., Cordova, K., Ypushima, A., Estela-Villar, E., Quintana-Criollo, C., Estacio, D., & Rodríguez, J. L. (2023). Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells. Toxics, 11(10), 839. https://doi.org/10.3390/toxics11100839
NO 2022 Descuento MDPI
NO Universidad Intercultural de la Amazonia VPI
DS Docta Complutense
RD 17 abr 2025