RT Journal Article
T1 In Vitro Destruction of Streptococcus pneumoniae Biofilms with Bacterial and Phage Peptidoglycan Hydrolases
A1 Domenech Lucas, Miriam
A1 García, Ernesto
A1 Moscoso, Miriam
AB Host- and phage-coded cell wall hydrolases have been used to fight Streptococcus pneumoniae growing as planktonic cells in vitro as well as in animal models. Until now, however, the usefulness of these enzymes in biofilm-grown pneumococci has gone untested. The antipneumococcal activity of different cell wall hydrolases produced by S. pneumoniae and a number of its phages was examined in an in vitro biofilm model. The major pneumococcal autolysin LytA, an N-acetylmuramoyl-l-alanine amidase, showed the greatest efficiency in disintegrating S. pneumoniae biofilms. The phage-encoded lysozymes Cpl-1 and Cpl-7 were also very efficient. Biofilms formed by the close pneumococcal relatives Streptococcus pseudopneumoniae and Streptococcus oralis were also destroyed by the phage endolysins but not by the S. pneumoniae autolysin LytA. A cooperative effect of LytA and Cpl-1 in the disintegration of S. pneumoniae biofilms was recorded.
PB American Society for Microbiology
SN 0066-4804
YR 2011
FD 2011-09
LK https://hdl.handle.net/20.500.14352/98914
UL https://hdl.handle.net/20.500.14352/98914
LA eng
NO Ministerio de Ciencia e Innovación
NO Dirección General de Investigación Científica y Técnica
DS Docta Complutense
RD 24 ago 2024