RT Journal Article
T1 Easy and Versatile Technique for the Preparation of Stable and Active Lipase-Based CLEA-Like Copolymers by Using Two Homofunctional Cross-Linking Agents: Application to the Preparation of Enantiopure Ibuprofen
A1 Khiari, Oussama
A1 Bouzemi, Nassima
A1 Sánchez Montero, José
A1 Alcántara León, Andrés Rafael
AB An easy and versatile method was designed and applied successfully to obtain access to lipase-based cross-linked-enzyme aggregate-like copolymers (CLEA-LCs) using one-pot, consecutive cross-linking steps using two types of homobifunctional cross-linkers (glutaraldehyde and putrescine), mediated with amine activation through pH alteration (pH jump) as a key step in the process. Six lipases were utilised in order to assess the effectiveness of the technique, in terms of immobilization yields, hydrolytic activities, thermal stability and application in kinetic resolution. A good retention of catalytic properties was found for all cases, together with an important thermal and storage stability improvement. Particularly, the CLEA-LCs derived from Candida rugosa lipase showed an outstanding behaviour in terms of thermostability and capability for catalysing the enantioselective hydrolysis of racemic ibuprofen ethyl ester, furnishing the eutomer (S)-ibuprofen with very high conversion and enantioselectivity.
PB MDPI
SN 1661-6596
YR 2023
FD 2023-09-04
LK https://hdl.handle.net/20.500.14352/92476
UL https://hdl.handle.net/20.500.14352/92476
LA eng
NO Khiari, O.; Bouzemi, N.; Sánchez-Montero, J.M.; Alcántara, A.R. Easy and Versatile Technique for the Preparation of Stable and Active Lipase-Based CLEA-like Copolymers by Using Two Homofunctional Cross-Linking Agents: Application to the Preparation of Enantiopure Ibuprofen. Int. J. Mol. Sci. 2023, 24, 13664. https://doi.org/10.3390/ijms241713664
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 20 may 2025