RT Book, Section
T1 Sample Processing for Metaproteomic Analysis of Human Gut Microbiota
A1 Monteoliva Díaz, Lucía
A1 Gil, Concha
A1 García Durán, Carmen
A1 Martínez López, Raquel María
A2 Springer, 
AB Human gut microbiota can be studied through the characterization of microorganisms present in feces. Metaproteomics has arisen as a good approach to investigate this vast community. However, the processing of fecal samples in order to obtain the largest number of proteins from gut microbiota to be subsequently analyzed by means of metaproteomics is a challenge. Here we describe a protocol to approach this task. It includes two main steps: the first step of humectation and dispersion of the feces, followed by the separation of microorganisms from other fecal components such as roughage and food debris, and the second step in which microbial cells are broken up and microbiota proteins recovered for MS analysis. Detailed procedures for sample preparation, protein extraction, trypsin digestion, and mass spectrometry analysis for gutmicrobiota samples are provided
PB Springer Nature
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/132002
UL https://hdl.handle.net/20.500.14352/132002
LA eng
NO García-Durán C, Martínez-López R, Monteoliva L, et al. Sample Processing for Metaproteomic Analysis of Human Gut Microbiota. In: Corrales FJ, Paradela A, Marcilla M, editors. Clinical Proteomics, vol. 2420, New York, NY: Springer US; 2022, p. 53–61. https://doi.org/10.1007/978-1-0716-1936-0_5
DS Docta Complutense
RD 25 feb 2026