RT Journal Article
T1 Histone Deacetylase Gene Expression Following BingeAlcohol Consumption in Rats and Humans
A1 López Moreno, José Antonio
A1 Marcos, Miguel
A1 Calleja Conde, Javier
A1 Echeverry-Alzate, Victor
A1 Buhler, Kora Mareen Katharina
A1 Costa-Alba, Pilar
A1 Bernardo, Edgar
A1 Laso, Francisco Javier
A1 Rodríguez De Fonseca, Fernando Antonio
A1 Nadal, Rose
A1 Viveros, María Paz
A1 Maldonado, Rafael
A1 Giné Domínguez, Elena
AB Background: Alcohol binge drinking is one of the most common patterns of excessive alcohol use and recent data would suggest that histone deacetylases (HDACs) gene expression profiling could be useful as a biomarker for psychiatric disorders.Methods: This study aimed to characterize the gene expression patterns of Hdac 1–11 in samples of rat peripheral blood, liver, heart, prefrontal cortex, and amygdala following repeated binge alcohol consumption and to determine the parallelism of Hdac gene expression between rats and humans in peripheral blood. To accomplish this goal, we examined Hdac gene expression following 1, 4, or 8 alcohol binges (3 g/kg, orally) in the rat, in patients who were admitted to the hospital emergency department for acute alcohol intoxication, and in rats trained in daily operant alcohol self-administration.Results: We primarily found that acute alcohol binging reduced gene expression (Hdac1–10) in the peripheral blood of alcohol-na€ıve rats and that this effect was attenuated following repeated alcohol binges. There was also a reduction of Hdac gene expression in the liver (Hdac2,4,5), whereas there was increased expression in the heart (Hdac1,7,8) and amygdala (Hdac1,2,5). Additionally, increased blood alcohol concentrations were measured in rat blood at 1 to 4 hours following repeated alcohol binging, and the only group that developed hepatic steotosis (fatty liver) were those animals exposed to 8 alcohol binge events. Finally, both binge consumption of alcohol in humans and daily operant alcohol self-administration in rats increased Hdac gene expression in peripheral blood.Conclusions: Our results suggest that increases in HDAC gene expression within the peripheral blood are associated with chronic alcohol consumption, whereas HDAC gene expression is reduced following initial exposure to alcohol.
PB Wiley
SN 0145-6008, ESSN: 1530-0277
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/23343
UL https://hdl.handle.net/20.500.14352/23343
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Fondo de Investigacion Sanitaria (Red de Trastornos Adictivos)-FEDER
NO European Foundation for Alcohol Research (Brussels)
DS Docta Complutense
RD 11 abr 2025