RT Journal Article
T1 High-Throughput CRISPR Screening in Hematological Neoplasms
A1 Ancos-Pintado, Raquel
A1 Bragado-García, Irene
A1 Morales, María Luz
A1 García-Vicente, Roberto
A1 Arroyo-Barea, Andrés
A1 Rodríguez-García, Alba
A1 Martínez López, Joaquín
A1 Linares Gómez, María
A1 Hernández Sánchez, María
AB CRISPR is becoming an indispensable tool in biological research, revolutionizing diverse fields of medical research and biotechnology. In the last few years, several CRISPR-based genome-targeting tools have been translated for the study of hematological neoplasms. However, there is a lack of reviews focused on the wide uses of this technology in hematology. Therefore, in this review, we summarize the main CRISPR-based approaches of high throughput screenings applied to this field. Here we explain several libraries and algorithms for analysis of CRISPR screens used in hematology, accompanied by the most relevant databases. Moreover, we focus on (1) the identification of novel modulator genes of drug resistance and efficacy, which could anticipate relapses in patients and (2) new therapeutic targets and synthetic lethal interactions. We also discuss the approaches to uncover novel biomarkers of malignant transformations and immune evasion mechanisms. We explain the current literature in the most common lymphoid and myeloid neoplasms using this tool. Then, we conclude with future directions, highlighting the importance of further gene candidate validation and the integration and harmonization of the data from CRISPR screening approaches.
PB MDPI
SN 2072-6694
YR 2022
FD 2022-07-25
LK https://hdl.handle.net/20.500.14352/101858
UL https://hdl.handle.net/20.500.14352/101858
LA eng
NO AECC (Ideas Semilla Award)
NO CRIS foundation
NO Spanish Society of Hematology
NO Formación de Profesorado Universitario
NO Ministry of Science, Innovation and Universities
NO Community of Madrid
DS Docta Complutense
RD 29 may 2025