RT Journal Article T1 Reproductive Cycle Stage Assessment in the Two-Toed Sloth (Choloepus Hoffmanni, Preliminary Results A1 Calvo Fernández, Cristina A1 Such, Roger A1 Gutiérrez Cepeda, Luna A1 Gómez Redondo, Isabel A1 García Vila, Encarnación A1 Cerdeira Lozano, Joaquín Vicente A1 Mayenco Aguirre, Ana María A1 Santiago Moreno, Julián A1 Hernández, Leticia A1 Sánchez Calabuig, María Jesús AB The number of two-toed sloths (Choloepus hoffmanni) has significantly decreased in the last years. Deepening the knowledge of this tropical mammal's reproductive physiology is essential to improve captive breeding within conservation programs for this species. However, several aspects of its reproductive biology remain unexplored and have not been described sufficiently. The aim of this work was to describe the estrous cycle and reproductive physiology of two adult female C. hoffmanni by vaginal cytology, appearance of the external genitalia, and behavior. Vaginal cytology assay showed that the average duration of the estrous cycle was 15.1 ± 4.53 d. Positive correlations (P < 0.05) were found between the peak presence of superficial cells (estrous phase) and four parameters: aggressive behavior, pursuing behavior, vulvar swelling, and vaginal discharge. This pilot study, conducted on just two animals, forms a basis for a study design that may be employed for a more comprehensive assessment of the two-toed sloth reproductive physiology and behavior. PB Bioone YR 2024 FD 2024-01-09 LK https://hdl.handle.net/20.500.14352/107090 UL https://hdl.handle.net/20.500.14352/107090 LA eng NO Calvo, C., Such, R., Gutiérrez-Cepeda, L., Gómez-Redondo, I., García-Vila, E., Cerdeira, J., ... & Sánchez-Calabuig, M. J. (2023). REPRODUCTIVE CYCLE STAGE ASSESSMENT IN THE TWO-TOED SLOTH (CHOLOEPUS HOFFMANNI), PRELIMINARY RESULTS. Departamento de Reproducción Animal​. DS Docta Complutense RD 31 jul 2025