RT Journal Article
T1 Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma
A1 Fernández Arrabal, José Antonio
A1 Salazar Corral, Juan José
A1 Hoz Montañana, María Rosa De
A1 Marco López, Eva María
A1 Martín Sánchez, Beatriz
A1 Flores Salguero, Elena
A1 García Martín, Elena Salobrar
A1 López Cuenca, Inés
A1 Barrios Sabador, Vicente
A1 Avilés Trigueros, Marcelino
A1 Valiente Soriano, Francisco Javier
A1 Miralles de Imperial-Ollero, Juan A.
A1 Vidal Sanz, Manuel
A1 Triviño Casado, Alberto
A1 Ramírez Sebastián, José Manuel
A1 López Gallardo, Meritxell
A1 Ramírez Sebastián, Ana Isabel
AB Signaling mediated by cytokines and chemokines is involved in glaucoma-associated neuroinflammation and in the damage of retinal ganglion cells (RGCs). Using multiplexed immunoassay and immunohistochemical techniques in a glaucoma mouse model at different time points after ocular hypertension (OHT), we analyzed (i) the expression of pro-inflammatory cytokines, anti-inflammatory cytokines, BDNF, VEGF, and fractalkine; and (ii) the number of Brn3a+ RGCs. In OHT eyes, there was an upregulation of (i) IFN-γ at days 3, 5, and 15; (ii) IL-4 at days 1, 3, 5, and 7 and IL-10 at days 3 and 5 (coinciding with downregulation of IL1-β at days 1, 5, and 7); (iii) IL-6 at days 1, 3, and 5; (iv) fractalkine and VEGF at day 1; and (v) BDNF at days 1, 3, 7, and 15. In contralateral eyes, there were (i) an upregulation of IL-1β at days 1 and 3 and a downregulation at day 7, coinciding with the downregulation of IL4 at days 3 and 5 and the upregulation at day 7; (ii) an upregulation of IL-6 at days 1, 5, and 7 and a downregulation at 15 days; (iii) an upregulation of IL-10 at days 3 and 7; and (iv) an upregulation of IL-17 at day 15. In OHT eyes, there was a reduction in the Brn3a+ RGCs number at days 3, 5, 7, and 15. OHT changes cytokine levels in both OHT and contralateral eyes at different time points after OHT induction, confirming the immune system involvement in glaucomatous neurodegeneration.
PB MDPI
SN 1422-0067
YR 2021
FD 2021-02-19
LK https://hdl.handle.net/20.500.14352/7890
UL https://hdl.handle.net/20.500.14352/7890
LA eng
NO Fernández Albarral, J. A., Salazar Corral, J. J., Hoz Motañana, M. R. et al. «Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma». International Journal of Molecular Sciences, vol. 22, n.o 4, febrero de 2021, p. 2066. DOI.org (Crossref), https://doi.org/10.3390/ijms22042066.
NO Este artículo pertenece al Nº especial "Retina Degeneration, Neuroprotection and Repair".
NO Ministerio de Economía, Comercio y Empresa (España)/Instituto de Salud Carlos III
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional/Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Fundación Séneca/Agencia de Ciencia y Tecnología Región de Murcia
NO Universidad Complutense de Madrid/Banco de Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 10 may 2025