RT Journal Article
T1 Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep.
A1 Casarrubios Molina, Laura
A1 Gómez Cerezo, María Natividad
A1 Sánchez Salcedo, Sandra
A1 Feito Castellano, María José
A1 Serrano, M.C.
A1 Saiz-Pardo, M.
A1 Ortega Menor, Lorena
A1 De Pablo, D.
A1 Díaz-Güemes, I.
A1 Fernández-Tomé, E.
A1 Enciso, S
A1 Portolés Pérez, María Teresa
A1 Sanchez-Margallo, F.M
A1 Arcos Navarrete, Daniel
A1 Vallet Regí, María Dulce Nombre
A1 Sanchez-Margallo, F. M.
AB Silicon-substituted hydroxyapatite (SiHA) macroporous scaffolds have been prepared by robocasting. In order to optimize their bone regeneration properties, we have manufactured these scaffolds presenting different microstructures: nanocrystalline and crystalline. Moreover, their surfaces have been decorated with vascular endothelial growth factor (VEGF) to evaluate the potential coupling between vascularization and bone regeneration. In vitro cell culture tests evidence that nanocrystalline SiHA hinders pre-osteblast proliferation, whereas the presence of VEGF enhances the biological functions of both endothelial cells and pre-osteoblasts. The bone regeneration capability has been evaluated using an osteoporotic sheep model. In vivo observations strongly correlate with in vitro cell culture tests. Those scaffolds made of nanocrystalline SiHA were colonized by fibrous tissue, promoted inflammatory response and forested osteoclast recruitment. These observations discard nanocystalline SiHA as a suitable material for bone regeneration purposes. On the contrary, those scaffolds made of crystalline SiHA and decorated with VEGF exhibited bone regeneration properties, with high ossification degree, thicker trabeculae and higher presence of osteoblasts and blood vessels. Considering these results, macroporous scaffolds made of SiHA and decorated with VEGF are suitable bone grafts for regeneration purposes, even in adverse pathological scenarios such as osteoporosis.
PB Elsevier
SN 1742-7061
YR 2019
FD 2019-10-31
LK https://hdl.handle.net/20.500.14352/5967
UL https://hdl.handle.net/20.500.14352/5967
LA eng
NO RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)
NO Unión Europea. H2020
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 22 jul 2024