RT Journal Article
T1 Cloning and heterologous production of hiracin JM79, a sec-dependent bacteriocin produced by enterococcus hirae DCH5, in lactic acid bacteria and pichia pastoris
A1 Sánchez, Jorge
A1 Borrero Del Pino, Juan
A1 Gómez Sala, Beatriz
A1 Basanta, Antonio
A1 Herranz Sorribes, Carmen
A1 Cintas Izarra, Luis Miguel
A1 Hernández Cruza, Pablo Elpidio
AB Hiracin JM79 (HirJM79), a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, was cloned and produced in Lactococcus lactis, Lactobacillus sakei, Enterococcus faecium, Enterococcus faecalis, and Pichia pastoris. For heterologous production of HirJM79 in lactic acid bacteria (LAB), the HirJM79 structural gene (hirJM79), with or without the HirJM79 immunity gene (hiriJM79), was cloned into the plasmid pMG36c under the control of the constitutive promoter P32 and into the plasmid pNZ8048 under the control of the inducible PNisA promoter. For the production of HirJM79 in P. pastoris, the gene encoding the mature HirJM79 protein was cloned into the pPICZαA expression vector. The recombinant plasmids permitted the production of biologically active HirJM79 in the supernatants of L. lactis IL1403, L. lactis NZ9000, L. sakei Lb790, E. faecalis JH2-2, and P. pastoris X-33, the coproduction of HirJM79 and nisin A in L. lactis DPC5598, and the coproduction of HirJM79 and enterocin P in E. faecium L50/14-2. All recombinant LAB produced larger quantities of HirJM79 than E. hirae DCH5, although the antimicrobial activities of most transformants were lower than that predicted from their production of HirJM79. The synthesis, processing, and secretion of HirJM79 proceed efficiently in recombinant LAB strains and P. pastoris. Copyright © 2008, American Society for Microbiology.
PB American Society for Microbiology
SN 0099-2240
SN 1098-5336
YR 2008
FD 2008-04-15
LK https://hdl.handle.net/20.500.14352/115213
UL https://hdl.handle.net/20.500.14352/115213
LA eng
NO Sánchez, J., Borrero, J., Gómez-Sala, B., Basanta, A., Herranz, C., Cintas, L. M., & Hernández, P. E. (2008). Cloning and heterologous production of hiracin JM79, a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, in lactic acid bacteria and Pichia pastoris. Applied and Environmental Microbiology, 74(8), 2471-2479. https://doi.org/10.1128/AEM.02559-07
NO Ministerio de Educación y Ciencia (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 17 abr 2025