RT Journal Article
T1 Prevention of Bacterial Adhesion to Zwitterionic Biocompatible Mesoporous Glasses
A1 Sánchez Salcedo, Sandra
A1 García Fontecha, Ana
A1 Vallet Regí, María Dulce Nombre
AB Novel materials, based on Mesoporous Bioactive Glasses(MBGs) in the ternary system SiO2-CaO-P2O5, decorated with (3-aminopropyl)triethoxysilane (APTES) and subsequently with amino acid Lysine (Lys), by post-grafting method on the external surface of the glasses (named MBG-NH2 and MBG-Lys), are reported. The surface functionalization with organic groups did not damage the mesoporous network and their structural and textural properties were also preserved despite the high solubility of MBG matrices. The incorporation of Lys confers a zwitterionic nature to these MBG materials due to the presence of adjacent amine and carboxylic groups in the external surface. At physiologic pH, this coexistence of basic amine and carboxilic acid groups from anchored Lys provided zero surface charge named zwitterionic effect. This behaviour could give rise to potential applications of antibacterial adhesion.  Therefore, in order to assess the influence of zwitterionic nature in in vitro bacterial adhesion, studies were carried out with Staphylococcus aureus. It was demonstrated that the efficient interaction of these zwitterionic pairs onto the MBG surfaces reduced bacterial adhesion up to 99.9% compared to bare MBGs. In order to test the suitability of zwitterionic MBGs materials as bone grafts, their cytocompatibility was investigated in vitro with MC3T3-E1 preosteoblasts. These findings suggested that the proposed surface functionalization strategy provided MBG materials with notable antibacterial adhesion properties, hence making  these materials promising candidates for local bone infection therapy.
PB Elsevier
SN 1742-7061
YR 2017
FD 2017-05-05
LK https://hdl.handle.net/20.500.14352/17843
UL https://hdl.handle.net/20.500.14352/17843
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 30 dic 2025