RT Journal Article
T1 The cannabinoid WIN55212-2 promotes neural repair after neonatal hypoxia-ischemia
A1 Fernández López, David
A1 Pradillo Justo, Jesús Miguel
A1 García Yébenes, Isaac
A1 Martínez Orgado, José Antonio
A1 Moro Sánchez, María Ángeles
A1 Lizasoaín Hernández, Ignacio
AB Background and Purpose—The endocannabinoid system has been involved in the modulation of neural stem cells proliferation, survival and differentiation as well as in the generation of new oligodendrocyte progenitors in the postnatal brain. The present work aims to test the effect of the synthetic Type 1 and Type 2 cannabinoid receptor agonist WIN55212-2 on these processes in the context of neonatal rat brain hypoxia–ischemia (HI).Methods—P7 Wistar rats were subjected to HI and treated either with WIN55212-2 (1 mg/kg) or vehicle twice daily for 7 days after HI and euthanized at 1, 2, 7, 14, or 28 days to explore white matter injury progression and the neurogenic response in the subventricular zone after HI.Results—Our findings reveal that WIN55212-2 promotes remyelination of the injured external capsule, increasing the number of NG2+ early oligodendrocyte progenitors 7 days after HI in this area and the number of APC+ mature oligodendrocytes in the injured striatum 14 and 28 days after HI. WIN55212-2 also increases cell proliferation and protein expression of the neuroblast marker doublecortin in the subventricular zone 7 days after neonatal HI as well as the number of newly generated neuroblasts (5-bromodeoxyuridine+/doublecortin+ cells) in the ipsilateral striatum 14 days after HI.Conclusions—Our results suggest that the activation of the endocannabinoid system promotes white and gray matter recovery after neonatal HI injury.
PB Wolters Kluwer Health, Inc.
SN 1524-4628
YR 2010
FD 2010-11-29
LK https://hdl.handle.net/20.500.14352/117174
UL https://hdl.handle.net/20.500.14352/117174
LA eng
NO Fernández-López, D., Pradillo, J. M., García-Yébenes, I., Martínez-Orgado, J. A., Moro, M. A., & Lizasoain, I. (2010). The cannabinoid WIN55212-2 promotes neural repair after neonatal hypoxia-ischemia. Stroke, 41(12), 2956–2964. https://doi.org/10.1161/STROKEAHA.110.599357
DS Docta Complutense
RD 24 abr 2025