RT Journal Article
T1 The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB2 and adenosine receptors
A1 Castillo, A.
A1 Tolón, M. R.
A1 Fernández Ruiz, J.
A1 Romero, J.
A1 Martínez Orgado, José Antonio
AB To investigate the mechanisms involved in cannabidiol (CBD)-induced neuroprotection in hypoxic–ischemic (HI) immature brain, forebrain slices from newborn mice underwent oxygen and glucose deprivation in the presence of vehicle, or CBD alone or with selective antagonists of cannabinoid CB1 and CB2, and adenosine A1 and A2 receptors. CBD reduced acute (LDH efflux to the incubation medium) and apoptotic (caspase-9 concentration in tissue) HI brain damage by reducing glutamate and IL-6 concentration, and TNFα, COX-2, and iNOS expression. CBD effects were reversed by the CB2 antagonist AM630 and by the A2A antagonist SCH58261. The A1A antagonist DPCPX only counteracted the CBD reduction of glutamate release, while the CB1 antagonist SR141716 did not modify any effect of CBD. In conclusion, CBD induces robust neuroprotection in immature brain, by acting on some of the major mechanisms underlying HI cell death; these effects are mediated by CB2 and adenosine, mainly A2A, receptors.
PB Elsevier
SN 0969-9961
YR 2009
FD 2009-11-06
LK https://hdl.handle.net/20.500.14352/117122
UL https://hdl.handle.net/20.500.14352/117122
LA eng
NO Castillo, A., Tolón, M. R., Fernández-Ruiz, J., Romero, J., & Martinez-Orgado, J. (2010). The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB(2) and adenosine receptors. Neurobiology of disease, 37(2), 434–440. https://doi.org/10.1016/j.nbd.2009.10.023
NO CIBERNED
NO Ministerio de Ciencia e Innovación Tecnológica
NO Ministerio de Sanidad (España)
NO Comunidad Autónoma de Madrid
DS Docta Complutense
RD 22 ene 2026