%0 Journal Article
%A García Calvo, Estefanía
%A Estévez Sánchez, Héctor
%A Álvarez-Fernández García, Roberto
%A Sánchez-Díaz, Raquel
%A Lazcano, Juan José
%A Martín, Pilar
%A Luque García, José Luis
%T SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
%D 2025
%U https://hdl.handle.net/20.500.14352/121630
%X Chitosan-stabilized selenium nanoparticles (Ch-SeNPs) are promising agents for cancer therapy due to their unique physicochemical properties, including spherical morphology and uniform size distribution. This study investigates the molecular mechanisms underlying their antitumoral effects, with a focus on the nuclear proteome. Quantitative proteomic analysis revealed 343 nuclear proteins, 47 of which showed significant changes following Ch-SeNPs treatment. Key regulators such as CDK1 and CDC5 were implicated in cell cycle arrest and tumor suppression pathways. Ch-SeNPs also affected processes including mRNA metabolism and cytoskeleton organization. In addition, Ch-SeNPs significantly inhibited tumor growth in a murine melanoma model, supporting their therapeutic potential.
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