RT Journal Article
T1 Study of the Association between Genotypic Potential and Linoleic Acid Tolerance with Microbial Production of Conjugated Linoleic Acid
A1 Fontes, Ana Luiza
A1 Leão Pimentel, Lígia
A1 Calzada Gómez, Javier
A1 Salsinha, Ana Sofia
A1 Rodríguez Gómez, Juan Miguel
A1 Gomes, Ana Maria
A1 Arqués Orobón, Juan Luis
A1 Rodríguez Alcalá, Luis Miguel
AB Probiotic bacteria have been shown to produce the bioactive conjugated linoleic acid (CLA) from linoleic acid (LA), this being associated with a detoxifying mechanism. Primers employed in genotypic selection are generally species-specific. Therefore, this work aimed to identify CLA producers by previously screening genes encoding enzymes involved in LA isomerization with primers designed based on conserved motifs among different species. Moreover, it was intended to evaluate the relation of LA tolerance with CLA production potential. Genotypically positive strains were further cultured with LA and CLA quantified. At least one of the screened genes was detected in 39 out of 85 strains, where designed primers allowed the identification of more potential producers than reported ones. The LA tolerance (<1–5 mg/mL) was shown to be independent of genotypical positivity/negativity. Only four strains exhibited CLA production (4.94–312.39 μg/mL). Thus, primers targeting conserved motifs are more efficient in the selection of potential CLA producers, however, this identification cannot rely solely on genotypic screening and LA tolerance.
PB American Chemical Society
YR 2023
FD 2023-12-15
LK https://hdl.handle.net/20.500.14352/104475
UL https://hdl.handle.net/20.500.14352/104475
LA eng
NO Ana Luiza Fontes, Lígia Leão Pimentel, Javier Calzada, Ana Sofia Salsinha, Juan Miguel Rodríguez, Ana Maria Gomes, Juan L. Arqués, and Luis Miguel Rodríguez-Alcalá. Study of the Association between Genotypic Potential and Linoleic Acid Tolerance with Microbial Production of Conjugated Linoleic Acid. ACS Food Science & Technology 2023 3 (12), 2199-2207 DOI: 10.1021/acsfoodscitech.3c00399
NO Fundação para a Ciência e a Tecnologia (Portugal)
DS Docta Complutense
RD 25 abr 2025