RT Journal Article
T1 Engineering a multifunctional hybrid polymer-hydrogel nanocomposite for high-sensitivity, anti-fouling electrochemical immunodetection in early cancer diagnosis
A1 Serafín González-Carrato, Verónica
A1 Cabrero Martín, Andrea
A1 Santiago, Sara
A1 Yildiz, Ceren
A1 Pedrero Muñoz, María
A1 Montero Calle, Ana
A1 Yazan, Zehra
A1 Dilek Eskiköy Bayraktepe, 
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB This work introduces a novel dual-component electrode modifier comprising gold nanoparticles (AuNPs) and a hydrated form of the poly(3,4-ethylenedioxythiophene) (hPEDOT) conducting polymer to enhance biomolecule immobilization and signal amplification in electrochemical immunosensing. The thiophene–AuNP interaction promotes electropolymerization, forming a citrate-doped matrix with carboxyl groups that enable covalent, oriented antibody anchoring. Moreover, the hydration of the polymeric scaffold enhances the biocompatibility and anti-fouling properties of the sensing interface. This multifunctional polymer–hydrogel nanocomposite was used to develop a disposable sandwich-type electrochemical immunoplatform for the determination of human endostatin (HE), a biomarker of colorectal cancer (CRC). The immunoplatform achieved a detection limit of 0.04 ng mL⁻1 and distinguished not only healthy individuals from early-stage CRC patients but also from those with premalignant colorectal lesions. These findings underscore the potential of this new multifunctional hybrid nanocomposite to improve the performance of electrochemical immunosensing, which is crucial for timely cancer diagnosis and intervention.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/123529
UL https://hdl.handle.net/20.500.14352/123529
LA eng
NO Verónica Serafín, Andrea Cabrero-Martín, Sara Santiago, Ceren Yildiz, María Pedrero, Ana Montero-Calle, Zehra Yazan, Dilek Eskiköy Bayraktepe, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Engineering a multifunctional hybrid polymer-hydrogel nanocomposite for high-sensitivity, anti-fouling electrochemical immunodetection in early cancer diagnosis, Sensors and Actuators B: Chemical, Volume 444, Part 2, 2025, 138480, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2025.138480.
NO Ministerio de Ciencia e Innovación español
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 4 sept 2025