RT Journal Article
T1 Integration of Transcriptomics and Metabolomics to Reveal the Molecular Mechanisms Underlying Rhodium Nanoparticles-Based Photodynamic Cancer Therapy
A1 Machuca Marcos, Andrés
A1 García Calvo, Estefanía
A1 Anunciação, Daniela S.
A1 Luque García, José Luis
AB Rhodium nanoparticles have recently been described as promising photosensitizers due to their low toxicity in the absence of near-infrared irradiation, but their high cytotoxicity when irradiated. Irradiation is usually carried out with a laser source, which allows the treatment to be localized in a specific area, thus avoiding undesirable side effects on healthy tissues. In this study, a multi-omics approach based on the combination of microarray-based transcriptomics and mass spectrometry-based untargeted and targeted metabolomics has provided a global picture of the molecular mechanisms underlying the anti-tumoral effect of rhodium nanoparticle-based photodynamic therapy. The results have shown the ability of these nanoparticles to promote apoptosis by suppressing or promoting anti- and pro-apoptotic factors, respectively, and by affecting the energy machinery of tumor cells, mainly blocking the β-oxidation, which is reflected in the accumulation of free fatty acids and in the decrease in ATP, ADP and NAD+ levels.
PB MDPI
SN 1999-4923
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/7465
UL https://hdl.handle.net/20.500.14352/7465
LA eng
NO Ministerio de Ciencia, Innovación
DS Docta Complutense
RD 13 abr 2025