RT Journal Article
T1 Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection
A1 Álvarez Sánchez, Julio
A1 Juan Ferré, Lucía De
A1 Bezos Garrido, Javier
A1 Romero Martínez, Beatriz
A1 Sáez, Jose Luis
A1 Reviriego Gordejo, F.J.
A1 Briones Dieste, Víctor
A1 Moreno Romo, Miguel Ángel
A1 Mateos García, Ana Isabel
A1 Domínguez Rodríguez, Lucas José
A1 Aranaz Martín, Alicia
AB Detection of infected animals is a key step in eradication programs of tuberculosis. Paratuberculosis infection has been demonstrated to compromise the specificity of the diagnostic tests. However, its effect on their sensitivity has not been clarified. In the present study, skin tests and the interferon-gamma (IFN-γ) assay were evaluated in a goat flock (n = 177) with a mixed tuberculosis–paratuberculosis infection in order to assess the possible effect of paratuberculosis on their sensitivity. Culture of mycobacteria was performed as the gold standard to determine the true infection status. All techniques showed lower sensitivities than previously described; the single intradermal tuberculin (SIT) test and the IFN-γ assay detected 71% (62.4–78.6, 95% C.I.) of the infected animals; the single intradermal cervical comparative tuberculin (SICCT) test detected only 42.7% (34.1–51.7, 95% C.I.) of infected animals. The highest level of sensitivity was obtained when SIT test and IFN-γ assay were combined in parallel (90.8%, 84.5–95.2, 95% C.I.). Sensitivities of the tests were also assessed by comparing animals suffering tuberculosis and animals with a mixed infection; tests were found to be more effective in the former group. Paratuberculosis seems to have a major effect in the sensitivity of the diagnostic tests under study, and therefore must be taken into account; in particular, the use of the SICCT test should be questioned when both tuberculosis and paratuberculosis are present.
PB ELSEVIER SCI LTD
SN 0378-1135
YR 2008
FD 2008-04
LK https://hdl.handle.net/20.500.14352/93528
UL https://hdl.handle.net/20.500.14352/93528
LA eng
NO Alvarez, J., de Juan, L., Bezos, J., Romero, B., Sáez, J. L., Reviriego Gordejo, F. J., Briones, V., Moreno, M. A., Mateos, A., Domínguez, L., & Aranaz, A. (2008). Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection. Veterinary microbiology, 128(1-2), 72–80. https://doi.org/10.1016/j.vetmic.2007.08.034
NO 2007 Elsevier B.V. All rights reserved.Financiación: AGL2004-08092 of the Spanish Ministry of Science; ParaTBTools (STREP 23106) of the European Union. Julio Álvarez: predoctoral grant assigned by the Spanish Ministry of Education and Culture.
NO European Union
NO Ministerio de Ciencia y Tecnología
NO Ministerio de Educación y Cultura
DS Docta Complutense
RD 1 sept 2024