RT Journal Article
T1 Permeability modes in fluctuating lipid membranes with DNA-translocating pores
A1 Moleiro, L.H.
A1 Mell, M.
A1 Bocanegra, R.
A1 López-Montero, Iván
A1 Fouquet, P.
A1 Hellweg, Th.
A1 Carrascosa, J.L.
A1 Monroy Muñoz, Francisco
AB Membrane pores can significantly alter not only the permeation dynamics of biological membranes but also their elasticity. Large membrane pores able to transport macromolecular contents represent an interesting model to test theoretical predictions that assign active-like (non-equilibrium) behavior to the permeability contributions to the enhanced membrane fluctuations existing in permeable membranes [Maneville et al. Phys. Rev. Lett. 82, 4356 (1999)]. Such high-amplitude active contributions arise from the forced transport of solvent and solutes through the open pores, which becomes even dominant at large permeability. In this paper, we present a detailed experimental analysis of the active shape fluctuations that appear in highly permeable lipid vesicles with large macromolecular pores inserted in the lipid membrane, which are a consequence of transport permeability events occurred in an osmotic gradient. The experimental results are found in quantitative agreement with theory, showing a remarkable dependence with the density of membrane pores and giving account of mechanical compliances and permeability rates that are compatible with the large size of the membrane pore considered. The presence of individual permeation events has been detected in the fluctuation time-series, from which a stochastic distribution of the permeation events compatible with a shot-noise has been deduced. The nonequilibrium character of the membrane fluctuations in a permeation field, even if the membrane pores are mere passive transporters, is clearly demonstrated. Finally, a bio-nano-technology outlook of the proposed synthetic concept is given on the context of prospective uses as active membrane DNA-pores exploitable in gen-delivery applications based on lipid vesicles.
PB Elsevier
SN 0001-8686
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/19025
UL https://hdl.handle.net/20.500.14352/19025
LA eng
NO The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 10 abr 2025