RT Journal Article
T1 Metabolomic response of osteosarcoma cells to nanographene oxide-mediated hyperthermia.
A1 Cicuéndez Maroto, Mónica
A1 Flores, Joana
A1 Oliveira, Helena
A1 Portolés Pérez, María Teresa
A1 Vallet Regí, María Dulce Nombre
A1 Vila, Mercedes
A1 Duarte, Lola F
AB Nanographene oxide  (nGO)-mediated hyperthermia has been  increasingly investigated as a localized, minimally invasive anticancer therapeutic approach. Near  InfraRed (NIR)  light  irradiation for  inducing hyperthermia  is particularly  attractive,  because biological systems   mostly   lack   chromophores  that   absorb  in  this   spectral window, facilitating the  selective heating and  destruction of cells  which have  internalized the  NIR absorbing- nanomaterials. However, little  is known about biological effects accompanying nGO-mediated hyperthermia at cellular and molecular levels.  In this work,  well-characterized pegylated nGO sheets  with  a hydrodynamic size of 300 nm were  incubated with  human Saos-2 osteosarcoma cells for 24 h and their internalization verified by flow cytometry and  confocal microscopy. No effect on cell viability was  observed after  nGO uptake by Saos-2  cells. However, a  proliferation delay  was  observed due  to  the  presence of nGO  sheets  in  the  cytoplasm. 1H NMR metabolomics was employed to screen for changes in the metabolic profile of cells, as this could  help  to improve understanding of cellular responses to  nanomaterials and  provide new  endpoint markers of effect. Cells inter- nalizing nGO sheets  showed noticeable changes in several metabolites compared to control cells, including decreased levels  of several amino acids,  taurine and  creatine and  increased levels  of phosphocholine and  ur- idine/adenosine nucleotides. After NIR irradiation, cells showed decreases in glutamate and uridine nucleotides, together with  increases in glycerophosphocholine and  adenosine monophosphate. Overall, this study  has shown that  the  cellular metabolome sensitively responded to nGO exposure and  nGO-mediated hyperthermia and  that NMR metabolomics is a powerful tool  to investigate treatment responses.
PB Elsevier
SN 0928-4931
YR 2018
FD 2018-05-18
LK https://hdl.handle.net/20.500.14352/12187
UL https://hdl.handle.net/20.500.14352/12187
LA eng
NO RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea. H2020
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 6 abr 2025