RT Journal Article
T1 Fractal analysis to assess the differentiation state of oligodendroglia in culture
A1 Pascual-Guerra, J.
A1 Torres-Rico, M.
A1 Paíno, C.L.
A1 Rodríguez Navarro, José Antonio
A1 García, A.G.
AB BackgroundOligodendroglial development is accompanied by increased cell complexity. A simple and cost-effective evaluation of the pro-myelinating activity of different drugs and/or treatments would be of great interest. In cultured oligodendroglia, an evaluation of the pro-myelinating activity of different drugs and/or treatments can be achieved through fractal analysis, which allows measuring cell complexity.New MethodFractal dimension was assessed in two O4+ cell types (neural stem cell-derived and lineage-converted adipose tissue mesenchymal cells) under proliferating or differentiating conditions.Comparison with Existing MethodsThis analysis, which was originally developed to analyze microglia, assigns a quantitative value (fractal dimension) to cellular profiles, obtaining higher coefficients as cells increase in size and arborizations instead of mRNA or protein quantification of mature oligodendroglial markers, such as MBP, MAG, O1 or PLP1/DM20.ResultsThis article describes a methodology to perform fractal analysis in immunofluorescent images of O4-positive (O4+) oligodendroglia using the FracLac plugin of ImageJ software. Pro-myelinating drug Benztropine-treated O4+ cells exhibit higher fractal dimension than control group.ConclusionsThe results demonstrated the effectiveness and sensitivity of the fractal dimension coefficient provided by FracLac software to assess the effects of treatments on oligodendroglial differentiation
PB Elsevier
SN 0165-0270
YR 2024
FD 2024-12-07
LK https://hdl.handle.net/20.500.14352/118165
UL https://hdl.handle.net/20.500.14352/118165
LA eng
NO Pascual-Guerra, J., Torres-Rico, M., Paíno, C. L., Rodríguez-Navarro, J. A., & García, A. G. (2025). Fractal analysis to assess the differentiation state of oligodendroglia in culture. Journal of Neuroscience Methods, 415, 110336. https://doi.org/10.1016/j.jneumeth.2024.110336
NO This work was supported by ISCIII [CP13–00234, CP19–0010]; MINECO [SAF2013–45570-R, SAF2016–78666R], FEDER; Project NDG09/014 of Agencia Laín Entralgo; Project VEXEM; IRYCIS; European Social Fund through Programa Operativo de Empleo Juvenil and Iniciativa de Empleo Juvenil (YEI) of Comunidad de Madrid [PEJ16/MED/AI-1153, PEJD-2018-PRE/SAL-8532].
NO Instituto de Salud Carlos III
NO Ministerio de Economía y Competitividad (España)
NO Agencia Laín Entralgo
NO Comunidad de Madrid
DS Docta Complutense
RD 18 abr 2025