RT Journal Article
T1 Dairy and plant based protein beverages: In vitro digestion behaviour and effect on intestinal barrier biomarkers
A1 Arranz Gutiérrez, Elena María
A1 Segat, Annalisa
A1 Velayos, Gemma
A1 Flynn, Cal
A1 Brodkorb, André
A1 Giblin, Linda
AB The consumer demand for protein-enriched food products continues to grow, in parallel with consumers' interest in plant based alternatives. The replacement of milk protein by plant protein is likely to be occur predominantly in prepared consumer foods such as nutritional beverages. This study aimed to compare and contrast powder beverages formulated with commercially available dairy versus plant ingredients in terms of protein digestion and gut barrier health. After simulated static in vitro gastrointestinal digestion, the release of free amino acids increased for all model beverages. In addition, the majority of peptides present in digested beverages were < 0.8 kDa in size. Gastrointestinal digestion did not increase the degree of protein hydrolysis in beverages formulated with prehydrolysed milk protein, whey or pea ingredients. A 2 h permeability assessment of digested beverages across the intestinal barrier, using Caco-2/HT-29/MTX co-cultures, revealed reduced transcription of tight junction protein 1, claudin-1 and mucus protein 2 albeit gut barrier impedance was unchanged. IL-8 mRNA levels in cell monolayers was significantly increased with digested fluids treatment but even more so with digesta from hydrolysed milk protein beverage. Overall, the response observed on intestinal biomarkers with digested plant beverages was similar to dairy based beverages supporting the replacement of dairy with plant proteins in powder beverage formulations.
PB Elsevier
SN 0963-9969
YR 2023
FD 2023-04-07
LK https://hdl.handle.net/20.500.14352/112052
UL https://hdl.handle.net/20.500.14352/112052
LA eng
NO Elena Arranz, Annalisa Segat, Gemma Velayos, Cal Flynn, André Brodkorb, Linda Giblin, Dairy and plant based protein beverages: In vitro digestion behaviour and effect on intestinal barrier biomarkers, Food Research International, Volume 169, 2023, 112815, https://doi.org/10.1016/j.foodres.2023.112815.
NO 2024 Acuerdos transformativos CRUE
NO European Commission
NO Enterprise Ireland (Career-FIT)
NO Science Foundation Ireland (SFI)
NO Department of Agriculture, Food and Marine on behalf of the Govern ment of Ireland
DS Docta Complutense
RD 10 abr 2025