%0 Journal Article
%A Gil Dones, Félix
%A Brun, Alejandro
%A Wigdorovitz, Andrés
%A Catalá, Rafael
%A Martı́nez-Torrecuadrada, Jorge 
%A Casal, Ignacio
%A Salinas, Julio
%A Borca, Manuel 
%A Escribano, José 
%T High‐yield expression of a viral peptide vaccine in transgenic plants
%D 2001
%@ 0014-5793
%U https://hdl.handle.net/20.500.14352/94486
%X A high-yield production of a peptide vaccine in transgenic plants is described here. A 21-mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino-terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant-derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low-cost production of peptide vaccines.
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