RT Journal Article
T1 High‐yield expression of a viral peptide vaccine in transgenic plants
A1 Gil Dones, Félix
A1 Brun, Alejandro
A1 Wigdorovitz, Andrés
A1 Catalá, Rafael
A1 Martı́nez-Torrecuadrada, Jorge
A1 Casal, Ignacio
A1 Salinas, Julio
A1 Borca, Manuel
A1 Escribano, José
AB A high-yield production of a peptide vaccine in transgenic plants is described here. A 21-mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino-terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant-derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low-cost production of peptide vaccines.
PB Wiley
SN 0014-5793
YR 2001
FD 2001
LK https://hdl.handle.net/20.500.14352/94486
UL https://hdl.handle.net/20.500.14352/94486
LA eng
NO Gil, Félix, et al. «High‐yield Expression of a Viral Peptide Vaccine in Transgenic Plants». FEBS Letters, vol. 488, n.o 1-2, enero de 2001, pp. 13-17. https://doi.org/10.1016/S0014-5793(00)02405-4.
NO This work was supported by Grant  07B/0014/99 from Comunidad Autónoma de Madrid and by Grant BID802/OC-AR PID168 from SECYT-CONICET.
NO Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
NO Comunidad de Madrid
DS Docta Complutense
RD 21 abr 2025