RT Journal Article
T1 Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
A1 Pedrero Muñoz, María
A1 Manuel de Villena Rueda, Francisco Javier
A1 Muñoz San Martín, Cristina
A1 Campuzano Ruiz, Susana
A1 Garranzo Asensio, María
A1 Barderas Manchado, Rodrigo
A1 Pingarrón Carrazón, José Manuel
AB An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.
PB MDPI
SN 2079-6374
YR 2016
FD 2016-11-21
LK https://hdl.handle.net/20.500.14352/19176
UL https://hdl.handle.net/20.500.14352/19176
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 20 ago 2024