RT Journal Article
T1 Neuroprotective activity of selenium nanoparticles against the effect of amino acid enantiomers in Alzheimer’s disease
T2 Actividad neuroprotectora de las nanopartículas de selenio frente al efecto de los enantiómeros de aminoácidos en la enfermedad de Alzheimer
A1 Vicente Zurdo, David
A1 Rodríguez-Blázquez, Sandra
A1 Gómez Mejía, Esther
A1 Rosales Conrado, Noelia
A1 León González, María Eugenia De
A1 Madrid Albarrán, María Yolanda
AB Alzheimer’s disease (AD), the most prevalent neurodegenerative disease, is characterized by extracellular accumulation of amyloid-beta protein (Aβ), which is believed to be the very starting event of AD neurodegeneration. In this work, D-Phe, D-Ala, and D-Glu amino acids, which are the non-occurring enantiomeric form in the human body, and also D-Asp and DL-SeMet, have proved to be amyloidogenic regarding Aβ42 aggregation in TEM studies. These amyloidogenic amino acid enantiomers also widened Aβ42 fibrils up to 437% regarding Aβ42 alone, suggesting that Aβ42 aggregation is enantiomerically dependent. To inhibit enantiomeric-induced amyloid aggregation, selenium nanoparticles stabilized with chitosan (Ch-SeNPs) were successfully synthesized and employed. Thus, Ch-SeNPs reduced and even completely inhibited Aβ42 aggregation produced in the presence of some amino acid enantiomers. In addition, through UV–Vis spectroscopy and fluorescence studies, it was deduced that Ch-SeNPs were able to interact differently with amino acids depending on their enantiomeric form. On the other hand, antioxidant properties of amino acid enantiomers were evaluated by DPPH and TBARS assays, with Tyr enantiomers being the only ones showing antioxidant effect. All spectroscopic data were statistically analysed through experimental design and response surface analysis, showing that the interaction between the Ch-SeNPs and the amino acids studied was enantioselective and allowing, in some cases, to establish the concentration ratios in which this interaction is maximum.
PB Springer Heidelberg
SN 1618-2642
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/92847
UL https://hdl.handle.net/20.500.14352/92847
LA eng
NO Vicente-Zurdo, D., Rodríguez-Blázquez, S., Gómez-Mejía, E. et al. Neuroprotective activity of selenium nanoparticles against the effect of amino acid enantiomers in Alzheimer’s disease. Anal Bioanal Chem 414, 7573–7584 (2022). https://doi.org/10.1007/s00216-022-04285-z
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
NO European Commission
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 23 jul 2024