RT Journal Article
T1 Impact of Vitamin D Deficit on the Rat Gut Microbiome
A1 Robles-Vera, Iñaki
A1 Callejo Arranz, María
A1 Ramos, Ricardo
A1 Duarte, Juan
A1 Pérez Vizcaíno, Francisco
AB Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.
PB MDPI
SN 2072-6643
YR 2019
FD 2019-10-24
LK https://hdl.handle.net/20.500.14352/8205
UL https://hdl.handle.net/20.500.14352/8205
LA eng
NO Ministerio de Economía y Competitividad (MINECO)/FEDER
DS Docta Complutense
RD 2 may 2024