RT Journal Article T1 Effect of Melatonin on Redox Enzymes Daily Gene Expression in Perirenal and Subcutaneous Adipose Tissue of a Diet Induced Obesity Model A1 Fernández Mateos, María Del Pilar A1 Cano Barquilla, María Pilar A1 Jiménez Ortega, Vanesa A1 Virto Ruiz, Leire A1 Pérez De Miguelsanz, María Juliana A1 Esquifino, Ana I. AB Increased adiposity is related to oxidative stress, inflammation and metabolic disorders. Our group has shown that melatonin totally or partially prevents the alterations that obesity causes in some neuroendocrine and inflammatory parameters indicative of oxidative stress. This study analyzes the effects of HFD on the relative gene expression of several redox balance enzymes on adult male Wistar rats subcutaneous (SAT) and perirenal adipose tissue (PRAT) and the possible preventive role of melatonin. Three experimental groups were established: control, high fat diet (HFD) and HFD plus 25 μg/mL melatonin in tap water. After 11 weeks, animals were sacrificed at 09:00 a.m. and 01:00 a.m. and PRAT and SAT were collected for selected redox enzymes qRT-PCR. Differential expression of redox enzyme genes, except for SODMn, GPx and catalase, was observed in the control group as a function of fat depot. HFD causes the disappearance of the temporal changes in the expression of the genes studied in the two fat depots analyzed. PRAT seems to be more sensitive than SAT to increased oxidative stress induced by obesity. Melatonin combined with a HFD intake, partially prevents the effects of the HFD on the gene expression of the redox enzymes. According to our results, melatonin selectively prevents changes in the relative gene expression of redox enzymes in PRAT and SAT of animals fed an HFD. PB MDPI SN 1422-0067 YR 2023 FD 2023-01-04 LK https://hdl.handle.net/20.500.14352/104895 UL https://hdl.handle.net/20.500.14352/104895 LA eng NO Fernández-Mateos, P.; Cano-Barquilla, P.; Jiménez-Ortega, V.; Virto, L.; Pérez-Miguelsanz, J.; Esquifino, A.I. Effect of Melatonin on Redox Enzymes Daily Gene Expression in Perirenal and Subcutaneous Adipose Tissue of a Diet Induced Obesity Model. Int. J. Mol. Sci. 2023, 24, 960. https://doi.org/10.3390/ijms24020960 DS Docta Complutense RD 8 abr 2025