%0 Journal Article
%A Pérez Sancho, Marta
%A García-Seco Romero, María Teresa
%A Arrogante, L
%A García, N
%A García Benzaquén, Nerea
%A Martínez Alares, Irene
%A Díez Guerrier, Alberto Antoine
%A Perales, A
%A Goyache Goñi, Joaquín
%A Domínguez Rodríguez, Lucas José
%A Álvarez Sánchez, Julio
%T Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples
%D 2013
%@ 0034-5288
%U https://hdl.handle.net/20.500.14352/93391
%X DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.
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