RT Journal Article
T1 Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples
A1 Pérez Sancho, Marta
A1 García-Seco Romero, María Teresa
A1 Arrogante, Lorena
A1 García, Nerea
A1 García Benzaquén, Nerea
A1 Martínez Alares, Irene
A1 Díez Guerrier, Alberto Antoine
A1 Perales, Agustina
A1 Goyache Goñi, Joaquín
A1 Domínguez Rodríguez, Lucas José
A1 Álvarez Sánchez, Julio
AB DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.
PB Elsevier
SN 0034-5288
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/93391
UL https://hdl.handle.net/20.500.14352/93391
LA eng
NO Pérez-Sancho, M., García-Seco, T., Arrogante, L., García, N., Martínez, I., Diez-Guerrier, A., Perales, A., Goyache, J., Domínguez, L., & Alvarez, J. (2013). Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples. Research in veterinary science, 95(2), 489–494. https://doi.org/10.1016/j.rvsc.2013.05.002
NO 2013 Elsevier Ltd. All rights reserved;Financiación: AGL-2009-12674 (Ministerio de Ciencia e Innovación). Consejería de Educación de la Comunidad de Madrid y Fondo Social Europeo. EU FP7 ANTIGONE Project Number 278976.
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
DS Docta Complutense
RD 10 abr 2025