RT Journal Article
T1 Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples
A1 Pérez Sancho, Marta
A1 García-Seco Romero, María Teresa
A1 Arrogante, L
A1 García, N
A1 García Benzaquén, Nerea
A1 Martínez Alares, Irene
A1 Díez Guerrier, Alberto Antoine
A1 Perales, A
A1 Goyache Goñi, Joaquín
A1 Domínguez Rodríguez, Lucas José
A1 Álvarez Sánchez, Julio
AB DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.
PB ELSEVIER SCI LTD
SN 0034-5288
YR 2013
FD 2013-10
LK https://hdl.handle.net/20.500.14352/93391
UL https://hdl.handle.net/20.500.14352/93391
LA eng
NO Pérez-Sancho, M., García-Seco, T., Arrogante, L., García, N., Martínez, I., Diez-Guerrier, A., Perales, A., Goyache, J., Domínguez, L., & Alvarez, J. (2013). Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples. Research in veterinary science, 95(2), 489–494. https://doi.org/10.1016/j.rvsc.2013.05.002
NO 2013 Elsevier Ltd. All rights reserved;Financiación: AGL-2009-12674 (Ministerio de Ciencia e Innovación). Consejería de Educación de la Comunidad de Madrid y Fondo Social Europeo. EU FP7 ANTIGONE Project Number 278976.
NO Comunidad de Madrid. IV Plan regional de investigación científica e innovación tecnológica, 2005-2008
NO Ministerio de Ciencia e Innovación. Proyecto  AGL-2009-12674
NO European Union. FP7 ANTIGONE Project Number 278976
NO Fondo Social Europeo
DS Docta Complutense
RD 10 sept 2024