RT Journal Article
T1 Influence of sex on chronic steroid-induced glaucoma: 24-Weeks follow-up study in rats
A1 García Herranz, David
A1 García Feijoo, Julián
A1 Bravo Osuna, Irene
A1 Herrero Vanrell, María Del Rocío
A1 Rodrigo MJ, 
A1 Martínez Rincón, T
A1 Subías, M
A1 Mendez-Martínez, S
A1 Pablo, L
A1 Munuera, I
A1 García-Martín, E
AB The objective was to evaluate ocular changes based on sex in steroid-induced glaucoma models in rats comparing healthy controls, over 24 weeks follow-up. Eighty-nine Long–Evans rats (38 males and 51 females) with steroid-induced glaucoma were analysed. Two steroid-induced glaucoma models were generated by injecting poly-co-lactic-glycolic acid microspheres loaded with dexamethasone (MMDEX model) and dexamethasone-fibronectin (MMDEXAFIBRO model) into the ocular anterior chamber. Intraocular pressure was measured by rebound tonometer Tonolab®. Neuroretinal function was analysed using dark- and light-adapted electroretinography (Roland consult® RETIanimal ERG), and structure was analysed using optical coherence tomography (OCT Spectralis, Heidelberg® Engineering) using Retina Posterior Pole, Retinal Nerve Fibre Layer and Ganglion Cell Layer protocols over 24 weeks. Males showed statistically (p < 0.05) higher intraocular pressure measurements. In both sexes and models neuroretinal thickness tended to decrease over time. In the MMDEX model, males showed higher IOP values and greatest percentage thickness loss in the Ganglion Cell Layer (p = 0.015). Females receiving MMDEXAFIBRO experienced large fluctuations in thickness, a higher percentage loss (on average) in Retina Posterior Pole (p = 0.035), Retinal Nerve Fibre Layer and Ganglion Cell Layer than aged-matched males, and the highest thickness loss rate by mmHg. Although no difference was found by sex in dark- and light-adapted electroretinography, increased amplitude in photopic negative response was found in MMDEX males and MMDEXAFIBRO females at 12 weeks. Although both glaucoma models used dexamethasone, different intraocular pressure and neuroretinal changes were observed depending on sex and other influential cofactors (fibronectin). Both sex and the induced glaucoma model influenced neuroretinal degeneration.
PB Elsevier
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/118036
UL https://hdl.handle.net/20.500.14352/118036
LA eng
NO Rodrigo MJ, Martinez-Rincon T, Subias M, Mendez-Martinez S, Garcia-Herranz D, Garcia-Feijoo J, Herrero-Vanrell R, Pablo L, Bravo-Osuna I, Munuera I, Garcia-Martin E. Influence of sex on chronic steroid-induced glaucoma: 24-Weeks follow-up study in rats. Experimental Eye Research 2024;238:109736. https://doi.org/10.1016/j.exer.2023.109736.-Osuna I, Munuera I, Garcia-Martin E. Influence of sex on chronic steroid-induced glaucoma: 24-Weeks follow-up study in rats. Experimental Eye Research 2024;238:109736. https://doi.org/10.1016/j.exer.2023.109736.
NO European Commission
NO Ministerio de Economía y Competitividad (España)
NO Instituto de Salud Carlos III (España)
NO European Regional Development Fund
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 24 abr 2025